Lucon-Xiccato Tyrone, Dadda Marco
Dipartimento di Psicologia Generale, Università di Padova, Italy.
Dipartimento di Psicologia Generale, Università di Padova, Italy.
Behav Processes. 2014 Jul;106:1-4. doi: 10.1016/j.beproc.2014.03.010. Epub 2014 Apr 4.
Zebrafish represents a very promising model to study memory function and impairment in vertebrates. The one-trial memory test has proven to be a reliable method to assess memory in mammals without the need for an extensive training procedure or the learning of a rule. To investigate whether such a test is suitable for zebrafish we observed adult fish in a modification of the original one-trial memory test developed for rats. Subjects were allowed to familiarize themselves with a new object for 25 min (exposure phase) and were then required to discriminate between the familiar object and a novel object that differed in shape and color (test phase). In both phases zebrafish showed a clear tendency to explore a new object, can memorize the characteristics of this object and use this information when a second object was presented irrespective of the delay (2, 6 or 24h) that separated the two phases. These results suggest that memory performance in fish could be also assessed using this very simple test.
斑马鱼是研究脊椎动物记忆功能和损伤的一个非常有前景的模型。单次试验记忆测试已被证明是评估哺乳动物记忆的可靠方法，无需广泛的训练程序或学习规则。为了研究这种测试是否适用于斑马鱼，我们在为大鼠开发的原始单次试验记忆测试的改进版本中观察成年鱼。让实验对象熟悉一个新物体25分钟（暴露阶段），然后要求它们区分熟悉的物体和形状与颜色不同的新物体（测试阶段）。在两个阶段中，斑马鱼都表现出明显倾向于探索新物体，能够记住该物体的特征，并在呈现第二个物体时使用此信息，而与分隔两个阶段的延迟时间（2、6或24小时）无关。这些结果表明，使用这种非常简单的测试也可以评估鱼类的记忆表现。
