Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
Genes (Basel). 2012 Nov 9;3(4):742-58. doi: 10.3390/genes3040742.
The mechanism of SV40 DNA replication is certainly not completely understood. The proteins that are necessary for replication have been known for quite some time, but how they work together to form a nanomachine capable of faithfully replicating the virus DNA is only partially understood. Some of the proteins involved have been crystallized and their 3D structures determined, and several EM reconstructions of SV40 T antigen have been generated. In addition, there is a fair amount of biochemical data that pinpoints the sites of interaction between various proteins. With this information, various models were assembled that show how the SV40 DNA replication nanomachine could be structured in three dimensional space. This process was aided by the use of a 3D docking program as well as fitting of structures. The advantage of the availability of these models is that they are experimentally testable and they provide an insight into how the replication machine could work. Another advantage is that it is possible to quickly compare newly published structures to the models in order to come up with improved models.
SV40 DNA 复制的机制当然还不完全清楚。很长一段时间以来，人们已经知道复制所必需的蛋白质，但它们如何协同工作形成一个能够忠实地复制病毒 DNA 的纳米机器，这只部分为人所知。一些涉及的蛋白质已经结晶，并确定了它们的 3D 结构，并且已经生成了几个 SV40 T 抗原的 EM 重建。此外，还有相当多的生化数据指出了各种蛋白质之间相互作用的位点。有了这些信息，就可以组装各种模型，展示 SV40 DNA 复制纳米机器如何在三维空间中构建。这个过程借助于 3D 对接程序和结构拟合得到了帮助。这些模型的优点是它们可以进行实验测试，并深入了解复制机器的工作原理。另一个优点是可以快速将新发表的结构与模型进行比较，以提出改进的模型。
