Kilincaslan Huseyin, Karatepe Hande Ozgun, Kilic Elif, Aydogdu Ibrahim, Gedik Ahmet Hakan, Olgac Vakur, Kucukkoc Mehmet
Department of Pediatric Surgery, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Department of Pediatrics, Zeynep Kamil Research and Education Hospital, Istanbul, Turkey.
Eur J Pediatr Surg. 2015 Jun;25(3):299-304. doi: 10.1055/s-0034-1371715. Epub 2014 Apr 4.
The aim of the article is to investigate the beneficial effects of methylene blue (MB) on the acute phase of experimental corrosive esophageal burns.
A total of 21 male Sprague-Dawley rats were allocated into three groups with an equal number in each group. In Group 1 (control), the esophageal lumen was irrigated with saline. In Groups 2 (untreated) and 3 (MB treatment), the distal esophagus was burned with 30% NaOH solution. In Group 3, MB was applied locally on the distal esophageal lumen (30 mg/kg) 90 minutes after the burn. In all the groups, animals were humanely killed at the end of the 7th day. Distal esophageal specimens were obtained for histopathological and biochemical examinations.
MB increased the tissue superoxide dismutase levels and the total antioxidant status, whereas it decreased the levels of the total oxidant status with significant attenuation of histopathological damage.
MB might have reduced the tissue damage in corrosive esophagitis because of its antioxidant effects.
本文旨在研究亚甲蓝（MB）对实验性腐蚀性食管烧伤急性期的有益作用。
将21只雄性Sprague-Dawley大鼠平均分为三组。第1组（对照组）用生理盐水冲洗食管腔。第2组（未治疗组）和第3组（MB治疗组）用30%氢氧化钠溶液烧伤食管远端。第3组在烧伤90分钟后在食管远端腔局部应用MB（30mg/kg）。在所有组中，动物在第7天结束时被人道处死。获取食管远端标本进行组织病理学和生化检查。
MB提高了组织超氧化物歧化酶水平和总抗氧化状态，同时降低了总氧化剂状态水平，并显著减轻了组织病理学损伤。
由于其抗氧化作用，MB可能减轻了腐蚀性食管炎中的组织损伤。
