Elmas Onur, Cevik Muazzez, Demir Tuncer, Ketani Muzaffer A
Department of Physiology, Mugla Sıtkı Kocman University, Mugla, Turkey
Department of Pediatric Surgery, Faculty of Medicine, Harran University, Sanliurfa, Turkey.
Interact Cardiovasc Thorac Surg. 2014 Sep;19(3):351-6. doi: 10.1093/icvts/ivu185. Epub 2014 Jun 3.
Corrosive oesophagitis is a common health problem in children. Scar tissue can develop during the recovery period, and as a result, serious narrowing of the oesophagus can develop, in turn causing morbidity and mortality. In previous studies, it was argued that tamoxifen (TAM) may have antifibrotic effects beyond its oestrogen antagonist or agonist properties. We aimed to examine the possible effects of TAM on fibrosis and stricture formation, which are complications of corrosive oesophagitis.
Three study groups were formed as follows: a non-oesophageal burn group (NON-EB, n = 6), an oesophageal burn group (EB, n = 6) and an oesophageal burn + tamoxifen group (EB-TAM, n = 6). In the NON-EB rats, the oesophageal lumen was washed with 0.9% NaCl while, in the EB and EB-TAM rats, the distal oesophagus was burned with a 50% NaOH solution. After application of this solution to the EB-TAM group rats, 0.4 mg/kg/day of TAM was administered via gavage for 7 days. Twenty-two days later, the rat oesophagi were examined histopathologically for inflammation, granulation, collagen deposition and stenosis.
In the EB group rats, the inflammation, collagen deposition and stenosis scores increased compared with those of the other groups. In the EB-TAM group, these three scores were lower compared with those of the EB group rats, but higher compared with those of the NON-EB group rats. No significant difference was observed in the granulation scores between the EB and EB-TAM groups. It was also observed that the EB-TAM group rats gained more weight than those in the EB group.
According to the data obtained, TAM use prevents inflammation, collagenization and stricture development. TAM may be a useful medicine in the treatment of corrosive oesophagitis.
腐蚀性食管炎是儿童常见的健康问题。在恢复期可能会形成瘢痕组织，进而导致食管严重狭窄，从而引起发病和死亡。在先前的研究中，有人认为他莫昔芬（TAM）可能具有超出其雌激素拮抗剂或激动剂特性的抗纤维化作用。我们旨在研究TAM对腐蚀性食管炎并发症纤维化和狭窄形成的可能影响。
组成三个研究组如下：非食管烧伤组（NON-EB，n = 6）、食管烧伤组（EB，n = 6）和食管烧伤+他莫昔芬组（EB-TAM，n = 6）。在NON-EB大鼠中，用0.9%氯化钠冲洗食管腔，而在EB和EB-TAM大鼠中，用50%氢氧化钠溶液烧伤食管远端。在将该溶液应用于EB-TAM组大鼠后，通过灌胃给予0.4mg/kg/天的TAM，持续7天。22天后，对大鼠食管进行组织病理学检查，观察炎症、肉芽组织、胶原沉积和狭窄情况。
与其他组相比，EB组大鼠的炎症、胶原沉积和狭窄评分增加。在EB-TAM组中，这三个评分低于EB组大鼠，但高于NON-EB组大鼠。EB组和EB-TAM组之间的肉芽组织评分未观察到显著差异。还观察到EB-TAM组大鼠比EB组大鼠体重增加更多。
根据所获得的数据，使用TAM可预防炎症、胶原化和狭窄的发展。TAM可能是治疗腐蚀性食管炎的一种有用药物。
