Xu H-S, Zong H-L, Shang M, Ming X, Zhao J-P, Ma C, Cao L
Department of Neurosurgery, the Central Hospital of Xuzhou, Xuzhou Clinical School of Xuzhou Medical College, Affiliated Hospital of Southeast University, Xuzhou, Jiangsu, People's Republic of China.
Eur Rev Med Pharmacol Sci. 2014;18(6):828-32.
To study the effects of the miR-324-5p on the glioma cells proliferation via the targeted regulation of the glioma-associated oncogene 1.
The luciferase reporter gene was used to test whether the glioma-associated oncogene 1 was the target of the miR-324-5p microRNA. The glioma-associated oncogene 1 expression was detected by Western blot. The proliferation and cell cycle were evaluated by MTT assay and flow cytometry.
The glioma-associated oncogene 1 is a target of the miR-324-5p. An over-expressed miR-324-5p could reduce the cell survival rate and increase the G1/G0 phase rate in the glioma cell lines.
The miR-324-5p can inhibit proliferation of the glioma cells via the targeted regulation of the glioma-associated oncogene 1.
通过靶向调控胶质瘤相关癌基因1，研究miR-324-5p对胶质瘤细胞增殖的影响。
利用荧光素酶报告基因检测胶质瘤相关癌基因1是否为miR-324-5p微小RNA的靶点。通过蛋白质免疫印迹法检测胶质瘤相关癌基因1的表达。采用MTT法和流式细胞术评估细胞增殖和细胞周期。
胶质瘤相关癌基因1是miR-324-5p的靶点。过表达的miR-324-5p可降低胶质瘤细胞系的细胞存活率，并提高G1/G0期比例。
miR-324-5p可通过靶向调控胶质瘤相关癌基因1抑制胶质瘤细胞增殖。
