College of Marine Sciences, South China Agriculture University, Guangzhou 510642, China.
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA.
Int J Mol Sci. 2020 Sep 20;21(18):6895. doi: 10.3390/ijms21186895.
, a small benthic fish, belongs to the Cynoglossidae, Pleuronectiformes. It was rarely studied due to its low abundance and cryptical lifestyle. In order to understand the mitochondrial genome and the phylogeny in Cynoglossidae, the complete mitogenome of has been sequenced and analyzed for the first time. The total length is 16,425 bp, typically containing 37 genes with novel gene rearrangements. The tRNA- gene is inverted from the light to the heavy strand and translocated from the downstream of tRNA- gene to its upstream. The control region (CR) translocated downstream to the 3'-end of gene adjoining to inverted to tRNA- and left a 24 bp trace fragment in the original position. The phylogenetic trees were reconstructed by Bayesian inference (BI) and maximum likelihood (ML) methods based on the mitogenomic data of 32 tonguefish species and two outgroups. The results support the idea that Cynoglossidae is a monophyletic group and indicate that has the closest phylogenetic relationship with . By combining fossil records and mitogenome data, the time-calibrated evolutionary tree of families Cynoglossidae and Soleidae was firstly presented, and it was indicated that Cynoglossidae and Soleidae were differentiated from each other during Paleogene, and the evolutionary process of family Cynoglossidae covered the Quaternary, Neogene and Paleogene periods.
圆斑星鲽，一种小型底栖鱼类，属于舌鳎科，鲽形目。由于其数量稀少且生活方式隐秘，因此很少被研究。为了了解舌鳎科的线粒体基因组和系统发育关系，我们首次对圆斑星鲽的完整线粒体基因组进行了测序和分析。该基因组全长 16425bp，通常包含 37 个基因，具有新颖的基因重排。tRNA-基因从轻链到重链反转，并从 tRNA-基因的下游转移到其上游。控制区（CR）从 基因的下游转移到 3′端，与 tRNA-基因相邻并在原始位置留下 24 个碱基的痕迹片段。基于 32 种舌鳎科物种和两个外群的线粒体基因组数据，采用贝叶斯推断（BI）和最大似然（ML）方法构建了系统发育树。结果支持了舌鳎科是一个单系群的观点，并表明圆斑星鲽与 具有最密切的系统发育关系。通过结合化石记录和线粒体基因组数据，首次提出了舌鳎科和鳎科的时间校准进化树，表明舌鳎科和鳎科在古近纪就已经分化，并且舌鳎科的进化过程涵盖了第四纪、新近纪和古近纪。
