Rhoades R W, Chiaia N L, Hess P R, Miller M W
Department of Anatomy, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Piscataway 08854.
J Neurosci. 1988 Jul;8(7):2234-47. doi: 10.1523/JNEUROSCI.08-07-02234.1988.
The distributions of substance P-like immunoreactivity (SPLI) and leucine-enkephalin-like immunoreactivity (LENKLI) in subnucleus caudalis of normal adult rats were compared with those observed in the adult rats that sustained transection of the infraorbital (IO) nerve either on the day of birth or in adulthood. All immunocytochemical experiments in the neonatally nerve damaged rats were carried out at least 60 d after the nerve transection. In the animals that sustained nerve transections as adults, brains were processed for immunohistochemistry between 7 and 60 d after the lesions. In the rats that sustained IO nerve transections as adults, there was a transient reduction in the density of the SPLI in layers I and II of ipsilateral subnucleus caudalis. It was most apparent about 2 weeks after the nerve transection and returned to near normal values by 60 d after the lesion. In the rats that sustained IO nerve transections on the day of birth, there was no reduction in the density of SPLI in caudalis, and the band of staining on the deafferented side of the brain stem was actually 40% wider than that on the intact side. Neither neonatal nor adult IO nerve transection had appreciable effects upon the distribution of LENKLI in the rat's trigeminal brain-stem complex. In another series of experiments, rats that sustained neonatal transection of the IO nerve had this same nerve recut in adulthood. Twelve days after the second lesion, the brains of these animals were processed for SPLI. There was a marked reduction in the density of the staining for this peptide on the deafferented side. This last result is consistent with the interpretation that the increased distribution of SPLI in the neonatally nerve damaged rats is due, at least partially, to reorganization of primary afferents.
将正常成年大鼠尾侧亚核中P物质样免疫反应性（SPLI）和亮氨酸脑啡肽样免疫反应性（LENKLI）的分布与出生当天或成年期遭受眶下（IO）神经横断的成年大鼠中观察到的分布进行了比较。所有新生神经损伤大鼠的免疫细胞化学实验均在神经横断后至少60天进行。在成年期遭受神经横断的动物中，在损伤后7至60天对大脑进行免疫组织化学处理。在成年期遭受IO神经横断的大鼠中，同侧尾侧亚核I层和II层中SPLI的密度出现短暂降低。在神经横断后约2周最为明显，并在损伤后60天恢复到接近正常的值。在出生当天遭受IO神经横断的大鼠中，尾侧SPLI的密度没有降低，并且脑干去传入侧的染色带实际上比完整侧宽40%。新生期或成年期的IO神经横断对大鼠三叉神经脑干复合体中LENKLI的分布均无明显影响。在另一系列实验中，新生期遭受IO神经横断的大鼠在成年期再次切断同一神经。第二次损伤后12天，对这些动物的大脑进行SPLI处理。去传入侧该肽的染色密度明显降低。最后这一结果与以下解释一致，即新生期神经损伤大鼠中SPLI分布增加至少部分是由于初级传入神经的重组。
