White F A, Bennett-Clarke C A, Chiaia N L, Hoeflinger B F, Rhoades R W
Department of Anatomy, Medical College of Ohio, Toledo 43699.
Brain Res Dev Brain Res. 1993 Apr 16;72(2):314-20. doi: 10.1016/0165-3806(93)90199-k.
Immunocytochemistry was used to demonstrate the distribution of galanin immunoreactivity in the trigeminal (V) brainstem complex of normal rats and rats that sustained neonatal transection of the infraorbital nerve (ION, the V branch that supplies the mystacial vibrissae). In normal adult and perinatal (postnatal day [P-] 7) rats, there is very little galanin immunoreactivity in the rostral part of the V brainstem complex. However, there is dense immunoreactivity for this peptide in layers I and II of V subnucleus caudalis (SpC). There was a marked upregulation of galanin immunoreactivity at all levels of the V brainstem complex of P-7 rats that sustained ION transection on P-0. In V subnucleus interpolaris and the magnocellular part of SpC, this immunoreactivity occurred in clusters which had a pattern resembling that of the mystacial vibrissae. In animals examined at 30 days or more after ION transection, there were a few more galanin-immunoreactive fibers on the deafferented side of the brainstem than on the intact side, but there was no evidence of a vibrissae-related pattern. Our interpretation of these results is that galanin immunocytochemistry reveals a vibrissae-related pattern in the central arbors of axotomized primary afferents for at least 1 week after they have been disconnected from the periphery.
免疫细胞化学法用于显示正常大鼠以及新生期眶下神经（ION，支配触须的三叉神经V分支）横断大鼠三叉神经（V）脑干复合体中甘丙肽免疫反应性的分布。在正常成年大鼠和围产期（出生后第[P-]7天）大鼠中，V脑干复合体头端部分的甘丙肽免疫反应性非常低。然而，在三叉神经尾侧亚核（SpC）的I层和II层中，该肽有密集的免疫反应性。在出生后第0天接受ION横断的P-7大鼠的V脑干复合体所有水平上，甘丙肽免疫反应性均有明显上调。在三叉神经极间亚核和SpC的大细胞部分，这种免疫反应性呈簇状出现，其模式类似于触须。在ION横断后30天或更长时间检查的动物中，脑干去传入侧的甘丙肽免疫反应性纤维比完整侧略多，但没有与触须相关的模式证据。我们对这些结果的解释是，甘丙肽免疫细胞化学显示，在轴突切断的初级传入神经元的中枢分支中，至少在它们与外周断开连接后的1周内存在与触须相关的模式。
