用罗丹明 - 抑肽酶对细胞上类胰蛋白酶的抑制作用。
Inhibition of trypsin-like enzymes on cells with rhodamine-aprotinin.
作者信息
Steven F S, Griffin M M, Williams L A, Freemont A J, Maier H, Weidauer H
机构信息
Department of Biochemistry, School of Biological Sciences, University of Manchester, U.K.
出版信息
J Enzyme Inhib. 1989;2(4):295-303. doi: 10.3109/14756368909088482.
Aprotinin, a polypeptide inhibitor of trypsin-like enzymes, has been labelled with rhodamine. Rhodamine-aprotinin inhibits trypsin in free solution in an identical manner to aprotinin. Rhodamine-aprotinin binds to trypsin-like enzymes on cells in formaldehyde fixed wax embedded sections. This technique has been used to locate cells possessing trypsin-like enzymes by means of fluorescent microscopy. In the present study we have used this technique to locate tumour cells.
抑肽酶是一种胰蛋白酶样酶的多肽抑制剂，已用罗丹明进行标记。罗丹明 - 抑肽酶在游离溶液中抑制胰蛋白酶的方式与抑肽酶相同。在甲醛固定的石蜡包埋切片中，罗丹明 - 抑肽酶可与细胞上的胰蛋白酶样酶结合。这项技术已被用于通过荧光显微镜定位具有胰蛋白酶样酶的细胞。在本研究中，我们已使用该技术来定位肿瘤细胞。
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