Department of Botany, University of Rzeszow, Kolbuszowa, Poland.
Department of Plant Physiology, University of Rzeszow, Kolbuszowa, Poland.
Biochem Biophys Res Commun. 2014 May 2;447(2):285-91. doi: 10.1016/j.bbrc.2014.03.141. Epub 2014 Apr 4.
Plant cell suspension cultures represent good model systems applicable for both basic research and biotechnological purposes. Nevertheless, it is widely known that a prolonged in vitro cultivation of plant cells is associated with genetic and epigenetic instabilities, which may limit the usefulness of plant lines. In this study, the age-dependent epigenetic and physiological changes in an asynchronous Arabidopsis T87 cell culture were examined. A prolonged cultivation period was found to be correlated with a decrease in the proliferation rate and a simultaneous increase in the expression of senescence-associated genes, indicating that the aging process started at the late growth phase of the culture. In addition, increases in the heterochromatin-specific epigenetic markers, i.e., global DNA methylation, H3K9 dimethylation, and H3K27 trimethylation, were observed, suggesting the onset of chromatin condensation, a hallmark of the early stages of plant senescence. Although the number of live cells decreased with an increase in the age of the culture, the remaining viable cells retained a high potential to efficiently perform photosynthesis and did not exhibit any symptoms of photosystem II damage.
植物细胞悬浮培养是一种很好的模型系统，适用于基础研究和生物技术目的。然而，众所周知，植物细胞的长期体外培养与遗传和表观遗传不稳定性有关，这可能限制了植物系的用途。在这项研究中，我们研究了拟南芥 T87 细胞培养中与年龄相关的表观遗传和生理变化。研究发现，较长的培养时间与增殖率的降低和衰老相关基因表达的同时增加有关，这表明衰老过程始于培养的后期生长阶段。此外，观察到异染色质特异性表观遗传标记（即全局 DNA 甲基化、H3K9 二甲基化和 H3K27 三甲基化）的增加，这表明染色质凝聚的开始，这是植物衰老早期的一个标志。尽管随着培养年龄的增加，活细胞的数量减少，但剩余的存活细胞仍保持高效进行光合作用的高潜力，并且没有表现出任何光系统 II 损伤的症状。
