Pérez-Jiménez Margarita, Cantero-Navarro Elena, Pérez-Alfocea Francisco, Le-Disquet Isabel, Guivarc'h Anne, Cos-Terrer José
Departamento de Hortofruticultura, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/ Mayor s/n, 30150 La Alberca, Murcia, Spain.
Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, 30100 Murcia, Spain.
J Plant Physiol. 2014 May 1;171(8):619-24. doi: 10.1016/j.jplph.2014.01.006. Epub 2014 Mar 20.
The relationship between endogenous hormones content and the induction of somatic peach plant was studied. To induce multiple shoots from callus derived from the base of stem explants of the scion cultivars 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach×almond rootstocks 'Garnem' and 'GF677', propagated plants were cultured on Murashige and Skoog salts augmented with 0.1mgL(-1) of indolebutyric acid, 1mgL(-1) of 6-benzylaminopurine and 3% sucrose. The highest regeneration rate was obtained with the peach×almond rootstocks. Endogenous levels of abscisic acid (ABA), indole-3-acetic acid (IAA), zeatin (Z), zeatin riboside (ZR), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA) were analyzed in the organogenic callus. Lower levels of several hormones, namely Z, ZR, ABA, and ACC were found in the peach×almond rootstock compared to peach cultivars, while IAA and SA presented inconclusive returns. These results suggest that the difference in somatic organogenesis capacity observed in peach and peach×almond hybrids is markedly affected by the endogenous hormonal content of the studied genotypes.
研究了内源激素含量与桃体细胞植株诱导之间的关系。为了从接穗品种‘UFO - 3’、‘Flariba’和‘Alice Bigi’以及桃×扁桃砧木‘Garnem’和‘GF677’的茎外植体基部产生的愈伤组织中诱导出多个芽，将增殖的植株培养在添加了0.1mgL(-1)吲哚丁酸、1mgL(-1) 6 - 苄基腺嘌呤和3%蔗糖的Murashige和Skoog盐培养基上。桃×扁桃砧木获得了最高的再生率。分析了器官发生愈伤组织中脱落酸（ABA）、吲哚 - 3 - 乙酸（IAA）、玉米素（Z）、玉米素核苷（ZR）、乙烯前体1 - 氨基环丙烷 - 1 - 羧酸（ACC）、水杨酸（SA）和茉莉酸（JA）的内源水平。与桃品种相比，桃×扁桃砧木中几种激素的水平较低，即Z、ZR、ABA和ACC，而IAA和SA的结果不明确。这些结果表明，在桃和桃×扁桃杂种中观察到的体细胞器官发生能力差异明显受到所研究基因型内源激素含量的影响。
