作为双顺反子表达分子工具的内部核糖体进入位点的比较分析。
Comparative analysis of internal ribosomal entry sites as molecular tools for bicistronic expression.
作者信息
Sadikoglou Eldem, Daoutsali Elena, Petridou Eleni, Grigoriou Maria, Skavdis George
机构信息
Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus, Building 10, Dragana, Alexandroupolis 68100, Greece.
Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus, Building 10, Dragana, Alexandroupolis 68100, Greece.
出版信息
J Biotechnol. 2014 Jul 10;181:31-4. doi: 10.1016/j.jbiotec.2014.03.033. Epub 2014 Apr 4.
Internal ribosomal entry sites (IRESes) are sequences that drive cap-independent translation. They are found in some viral and cellular transcripts and they have been extensively used in both basic and applied research for the translation of two or more polypeptides from a single mRNA molecule in eukaryotic cells. Although the most widely used IRES comes from the encephalomyocarditis virus (EMCV), several other viral and cellular IRES elements have been identified and successfully used, including those of the human VCIP gene and the mouse Gtx gene. In this report we have compared the EMCV IRES with the VCIP and the Gtx IRESes, and we provide evidence that by using the EMCV IRES much higher levels of second cistron expression can be achieved.
内部核糖体进入位点（IRESes）是驱动不依赖帽子结构的翻译的序列。它们存在于一些病毒和细胞转录本中，并且已在基础研究和应用研究中广泛用于在真核细胞中从单个mRNA分子翻译两种或更多种多肽。尽管使用最广泛的IRES来自脑心肌炎病毒（EMCV），但已鉴定并成功使用了其他几种病毒和细胞IRES元件，包括人类VCIP基因和小鼠Gtx基因的IRES元件。在本报告中，我们将EMCV IRES与VCIP和Gtx IRES进行了比较，并提供证据表明，使用EMCV IRES可以实现更高水平的第二个顺反子表达。
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