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[一种测定生物活性纤连蛋白浓度的方法]

[A method of determining the concentration of biologically active fibronectin].

作者信息

Safina N A, Kharrasov A F, Zinkevich O D

出版信息

Lab Delo. 1989(4):27-30.

PMID:2470960
Abstract

The suggested method is based on the ability of fibronectin intact molecules to agglutinate gelatin-coated particles. Gelatin-coated particles have been prepared with the use of specially treated Staphylococcus epidermidis cells. The concentration of biologically active fibronectin has been measured using laser nephelometry and agglutination test in microtitre plates. The accuracy of the test is 4 mg/ml (laser nephelometer) and 0.1 mg/ml (agglutination test).

摘要

所建议的方法基于纤连蛋白完整分子凝集明胶包被颗粒的能力。明胶包被颗粒是利用经过特殊处理的表皮葡萄球菌细胞制备的。使用激光散射比浊法和微量滴定板凝集试验测定了生物活性纤连蛋白的浓度。该试验的准确度为4毫克/毫升(激光散射比浊仪)和0.1毫克/毫升(凝集试验)。

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