Potapov Iu N, Pashkova V S
Lab Delo. 1989(4):47-8.
The proliferative activity of human pulmonary carcinoma cells has been examined by autoradiography in 48 cultures in diffuse chambers, incubated with 3H thymidine under various conditions. 3H thymidine has been injected intraperitoneally to one group of animals, in another group the diffuse chambers have been removed from the animals and incubated in culture medium with 3H thymidine in the same concentration. The proliferation intensity of the cell cultures from the same patients has been virtually the same in both modes of incubation. This permits simplifying the autoradiography technique for such experiments, thus helping to avoid the difficulties connected with radioactive waste utilization.
在48个置于扩散小室中的培养物中，在各种条件下用³H胸腺嘧啶核苷进行孵育，通过放射自显影术检测了人肺癌细胞的增殖活性。对一组动物进行腹腔注射³H胸腺嘧啶核苷，另一组则将扩散小室从动物体内取出，在含有相同浓度³H胸腺嘧啶核苷的培养基中进行孵育。来自相同患者的细胞培养物在两种孵育方式下的增殖强度几乎相同。这使得此类实验的放射自显影技术得以简化，从而有助于避免与放射性废物处理相关的困难。
