Departamento de Genetica, Facultad de Veterinaria de Cordoba, Cordoba, España, ES.
Lasers Med Sci. 1998 Dec;13(4):260-4. doi: 10.1007/s101030050005.
Low power He-Ne laser irradiation was applied to immature bovine oocytes to investigate the incidence of unreduced oocytes (diploid oocytes) during the first meiotic division in vitro. Immature bovine oocytes from cows killed at a slaughterhouse were irradiated with He-Ne laser irradiation at 0.05 and 0.25 J/cm(2) exposures. An oocyte group was left untreated serving as the control group. All oocytes were matured in TCM-199 medium supplemented with 10% fetal calf serum (FCS). Unreduced oocyte percentages obtained in the irradiated oocytes group were significantly higher (p<0.01 and p<0.001, respectively) than those of the control group. Furthermore, the laser-treated oocytes showed a degeneration rate significantly higher (p<0.01 and p<0.001, respectively) than those observed in the control group. It is concluded that the application of He-Ne laser irradiation at 0.05 and 0.25 J/cm(2) exposures increased the incidence of unreduced oocytes and the percentage of degenerated oocytes during the in vitro meiosis process of immature bovine oocytes.
低强度氦氖激光照射对体外第一次减数分裂过程中未减数卵母细胞（二倍体卵母细胞）的发生率的影响。用 0.05 和 0.25 J/cm(2) 的氦氖激光照射从屠宰场宰杀的奶牛中获得的不成熟牛卵母细胞。一组卵母细胞未经处理作为对照组。所有卵母细胞均在添加 10%胎牛血清（FCS）的 TCM-199 培养基中成熟。照射组的未减数卵母细胞百分比明显高于对照组（分别为 p<0.01 和 p<0.001）。此外，激光处理的卵母细胞的退化率明显高于对照组（分别为 p<0.01 和 p<0.001）。结论：0.05 和 0.25 J/cm(2) 的氦氖激光照射增加了不成熟牛卵母细胞体外减数分裂过程中未减数卵母细胞的发生率和退化卵母细胞的百分比。
