Jiao Wei, Xie Li, Li Hailan, Lan Jiao, Mo Zhuning, Yang Ziji, Liu Fei, Xiao Ruiping, He Yunlei, Ye Luyi, Zhu Ziyan
Blood Transfusion of Guangxi Zhuang Autonomous Region People's Hospital, Guangxi 530021, P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2014 Apr;31(2):242-6. doi: 10.3760/cma.j.issn.1003-9406.2014.02.025.
To screen rare blood groups Fy(a-), s-, k-, Di(b-) and Js(b-) in an ethnic Zhuang population.
Sequence-specific primers were designed based on single nucleotide polymorphism (SNP) sites of blood group antigens Fy(b) and s. A specific multiplex PCR system I was established. Multiplex PCR system II was applied to detect alleles antigens Di(b), k, Js(b)1910 and Js(b) 2019 at the same time. The two systems was were used to screen for rare blood group antigens in 4490 randomly selected healthy donors of Guangxi Zhuang ethnic origin.
We successfully made the multiplex PCR system I. We detected the rare blood group antigens using the two PCR system. There are five Fy(a-), three s(-), two Di(b-) in 4490 Guangxi zhuang random samples. The multiplex PCR system I has achieved good accuracy and stability. With multiplex PCR systems I and II, 4490 samples were screened. Five Fy(a-), three s(-) and two Di(b-) samples were discovered.
Multiplex PCR is an effective methods, which can be used for high throughput screening of rare blood groups. The rare blood types of Guangxi Zhuang ethnic origin obtained through the screening can provide valuable information for compatible blood transfusion. Through screening we obtained precious rare blood type materials which can be used to improve the capability of compatible infusion and reduce the transfusion reactions.
在壮族人群中筛查罕见血型Fy(a-)、s-、k-、Di(b-)和Js(b-)。
根据血型抗原Fy(b)和s的单核苷酸多态性（SNP）位点设计序列特异性引物，建立特异性多重PCR系统I，应用多重PCR系统II同时检测等位基因抗原Di(b)、k、Js(b)1910和Js(b)2019。利用这两个系统对4490名随机选取的广西壮族健康献血者进行罕见血型抗原筛查。
成功构建多重PCR系统I，利用两个PCR系统检测罕见血型抗原。在4490份广西壮族随机样本中，发现5例Fy(a-)、3例s(-)、2例Di(b-)。多重PCR系统I具有良好的准确性和稳定性。利用多重PCR系统I和II对4490份样本进行筛查，发现5例Fy(a-)、3例s(-)和2例Di(b-)样本。
多重PCR是一种有效的方法，可用于高通量筛查罕见血型。通过筛查获得的广西壮族罕见血型可为相容性输血提供有价值的信息。通过筛查获得了珍贵的罕见血型材料，可用于提高相容性输注能力，减少输血反应。
