Lundkvist I, Portnoi D, Coutinho A
Unit for Applied Cell and Molecular Biology, Umeå University, Sweden.
Res Immunol. 1989 Jan;140(1):7-18. doi: 10.1016/0923-2494(89)90003-5.
Priming of adult responder mice with optimal immunogenic doses of dextran alpha, 1-6 results in reduced antibody responses to secondary antigenic challenge. We have now quantitated dextran-specific clonal precursors in the large and small B-lymphocyte compartments within several days or several months after priming with either dextran or antiidiotypic antibodies directed to a dominant idiotype of C57BL/6 mice which accounts for more than 50% of the antibody response. The results show that "secondary unresponsiveness" correlates with idiotype-directed depletion of the appropriate specificities from the immunocompetent resting B-cell pool.
用最佳免疫原剂量的α-1,6葡聚糖对成年反应小鼠进行初次免疫，会导致对二次抗原攻击的抗体反应减弱。我们现在已经对用葡聚糖或针对C57BL/6小鼠占抗体反应50%以上的显性独特型的抗独特型抗体进行初次免疫后几天或几个月内大小B淋巴细胞区室中葡聚糖特异性克隆前体进行了定量。结果表明，“二次无反应性”与从免疫活性静止B细胞库中针对独特型的适当特异性的耗竭相关。
