Stankovic C J, Heinemann S H, Delfino J M, Sigworth F J, Schreiber S L
Science. 1989 May 19;244(4906):813-7. doi: 10.1126/science.2471263.
The gramicidin A transmembrane channel is believed to consist of two head-to-head beta helices. Computer-generated models were used to formulate the structure of new single-chain channel molecules based on the gramicidin motif. The chemical synthesis of two tartaric acid-gramicidin A hybrids and single-channel analyses of their conducting properties are reported. These studies illustrate the rational design and synthesis of long-lived channels with tunable conductance properties and provide support for current molecular models of the natural (dimeric) gramicidin channel.
短杆菌肽A跨膜通道被认为由两个头对头的β螺旋组成。基于短杆菌肽基序，利用计算机生成的模型来构建新的单链通道分子的结构。本文报道了两种酒石酸 - 短杆菌肽A杂化物的化学合成及其导电性质的单通道分析。这些研究阐明了具有可调电导特性的长寿命通道的合理设计与合成，并为天然（二聚体）短杆菌肽通道的当前分子模型提供了支持。
