Yewdell J W, Bennink J R
Laboratory for Viral Diseases, National Institute of Allergy and Infectious Diseases, Rockville, MD 20852.
Science. 1989 Jun 2;244(4908):1072-5. doi: 10.1126/science.2471266.
Cytotoxic T lymphocytes (CTLs) recognize foreign antigens, including viral proteins, in association with major histocompatibility complex (MHC) class I molecules. Brefeldin A, a specific inhibitor of exocytosis, completely and reversibly inhibited the presentation of viral proteins, but not exogenous peptides, to MHC class I-restricted CTLs directed against influenza virus antigens. The effect of brefeldin A on antigen presentation correlated with its inhibition of intracellular transport of newly synthesized class I molecules. Brefeldin A is thus a specific inhibitor of antigen processing for class I-restricted T cell recognition. Its effect on antigen presentation supports the idea that exogenous peptide antigens associate with cell surface class I molecules, whereas protein antigens processed via the cytosolic route associate with nascent class I molecules before they leave the trans-Golgi complex.
细胞毒性T淋巴细胞（CTLs）识别与主要组织相容性复合体（MHC）I类分子相关的外来抗原，包括病毒蛋白。布雷菲德菌素A是一种胞吐作用的特异性抑制剂，它能完全且可逆地抑制病毒蛋白向针对流感病毒抗原的MHC I类限制性CTLs的呈递，但不抑制外源性肽的呈递。布雷菲德菌素A对抗原呈递的影响与其对新合成的I类分子细胞内转运的抑制作用相关。因此，布雷菲德菌素A是I类限制性T细胞识别的抗原加工的特异性抑制剂。它对抗原呈递的影响支持了这样一种观点，即外源性肽抗原与细胞表面I类分子结合，而通过胞质途径加工的蛋白质抗原在离开反式高尔基体复合体之前与新生的I类分子结合。
