Kundu A K, Sharma A K
Department of Botany, University of Calcutta, India.
Stain Technol. 1988 Nov;63(6):369-72. doi: 10.3109/10520298809107613.
Costus speciosus (Koenig) Sm. contains diosgenin, an important drug in family planning programs in India and underdeveloped countries. A simple, rapid method has been developed for in situ quantitation of diosgenin in this plant. The method is based on the formation of a suitable colored product of diosgenin in frozen sections of fresh material followed by determination of its optical density by in situ cytophotometry. The staining reagent is a combination of anisaldehyde, sulfuric acid and acetic acid. A positive correlation has been observed between cytophotometric diosgenin estimates and those derived from thin layer chromatography of extracts. The method is convenient for routine screening of plants for steroidal sapogenins such as diosgenin.
闭鞘姜（Costus speciosus (Koenig) Sm.）含有薯蓣皂苷元，这是印度及不发达国家计划生育项目中的一种重要药物。已开发出一种简单、快速的方法用于原位定量该植物中的薯蓣皂苷元。该方法基于在新鲜材料的冰冻切片中形成合适的薯蓣皂苷元有色产物，然后通过原位细胞光度测定法测定其光密度。染色试剂是茴香醛、硫酸和乙酸的组合。已观察到细胞光度法对薯蓣皂苷元的估计值与提取物薄层色谱法得出的估计值之间存在正相关。该方法便于对植物进行甾体皂苷元如薯蓣皂苷元的常规筛选。
