Sarkar M, Mahato S B
Anal Biochem. 1984 Jan;136(1):168-74. doi: 10.1016/0003-2697(84)90319-1.
Homogeneous enzyme immunoassay has been used as a tool for the determination of diosgenin and its glycosides in plants. Diosgenin antisera was found to inhibit the activity of diosgenin hemisuccinate-horseradish peroxidase conjugate which was reversed by the addition of free diosgenin or its glycosides. The increase of enzyme activity was proportional to the quantity of the hapten over a certain range of hapten concentration. Thus, a minimum of 2.5 micrograms/ml of diosgenin and 11.5 micrograms/ml of diosgenin glycosides could be determined by this method. The results were comparable with those obtained by high-performance liquid chromatography and gravimetric methods.
均相酶免疫测定法已被用作测定植物中薯蓣皂苷元及其糖苷的工具。发现薯蓣皂苷元抗血清可抑制半琥珀酸薯蓣皂苷元 - 辣根过氧化物酶结合物的活性，而加入游离薯蓣皂苷元或其糖苷可使其活性恢复。在一定的半抗原浓度范围内，酶活性的增加与半抗原的量成正比。因此，用该方法可测定的薯蓣皂苷元最低浓度为2.5微克/毫升，薯蓣皂苷元糖苷为11.5微克/毫升。结果与高效液相色谱法和重量法所得结果相当。
