CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia.
CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia.
Ultrason Sonochem. 2014 Nov;21(6):2084-91. doi: 10.1016/j.ultsonch.2014.03.013. Epub 2014 Mar 25.
Lignocellulosic biomass samples (wheat chaff) were pretreated by ultrasound (US) (40kHz/0.5Wcm(-2)/10min and 400kHz/0.5Wcm(-2)/10min applied sequentially) prior to digestion by enzyme extracts obtained from fermentation of the biomass with white rot fungi (Phanerochaete chrysosporium or Trametes sp.). The accessibility of the cellulosic components in wheat chaff was increased, as demonstrated by the increased concentration of sugars produced by exposure to the ultrasound treatment prior to enzyme addition. Pretreatment with ultrasound increased the concentration of lignin degradation products (guaiacol and syringol) obtained from wheat chaff after enzyme addition. In vitro digestibility of wheat chaff was also enhanced by the ultrasonics pretreatment in combination with treatment with enzyme extracts. Degradation was enhanced with the use of a mixture of the enzyme extracts compared to that for a single enzyme extract.
木质纤维素生物质样品（麦麸）先用超声（US）预处理（40kHz/0.5Wcm(-2)/10min 和 400kHz/0.5Wcm(-2)/10min 依次施加），然后再用白腐真菌（黄孢原毛平革菌或栓菌）发酵生物质获得的酶提取物进行酶解。超声处理可增加麦麸中纤维素成分的可及性，这可通过在添加酶之前暴露于超声处理而增加产生的糖的浓度来证明。用酶添加后，超声预处理可增加从麦麸中获得的木质素降解产物（愈创木酚和丁香酚）的浓度。超声预处理与酶提取物处理相结合还可增强麦麸的体外消化率。与使用单一酶提取物相比，使用酶提取物混合物可增强降解。
