Favorov M O, Gol'dberg E Z, Gurov A V, Zairov G K, Iashina T L
Vopr Virusol. 1989 Jan-Feb;34(1):47-50.
Observations of 416 patients with and 112 convalescents after non-A-non-B virus hepatitis (HnAnB) with fecal-oral mechanism of transmission were carried out in 1984-1986. An enzyme immunoassay (EIA) was developed for the detection of HnAnB antigen in faeces. The rate of this antigen detection varied from 9.1% to 34.6% in different areas. The HnAnB antigen is present in two areas of cesium chloride density gradient: the maximum at 1.39-1.40 g/cm3 and another peak in the zone of 1.18-1.23 g/cm3. The method of radioimmunoprecipitation followed by SDS-polyacrylamide gel analysis showed the presence of several polypeptides of HnAnB virus. Most clearly, protein 30 K was documented, also polypeptides 91-94 K and 48-57 K were demonstrated. The resistance of nucleic acid of HnAnB virus to RNase was tested. The results indicate that the isolated NA is likely to be DNA. The above data suggest that the agent inducing HnAnB belongs to the group of parvoviruses.
1984年至1986年期间，对416例非甲非乙型肝炎（HnAnB）患者及112例恢复期患者进行了观察，该型肝炎通过粪-口途径传播。开发了一种酶免疫测定法（EIA）用于检测粪便中的HnAnB抗原。该抗原的检出率在不同地区从9.1%到34.6%不等。HnAnB抗原存在于氯化铯密度梯度的两个区域：在1.39 - 1.40 g/cm³处有最大值，在1.18 - 1.23 g/cm³区域有另一个峰值。采用放射免疫沉淀法随后进行SDS - 聚丙烯酰胺凝胶分析的方法显示存在HnAnB病毒的几种多肽。最明显的是记录到了30K蛋白，也证实了91 - 94K和48 - 57K的多肽。对HnAnB病毒核酸的核糖核酸酶抗性进行了测试。结果表明分离出的核酸很可能是DNA。上述数据表明，引发HnAnB的病原体属于细小病毒组。
