Seelig R, Liehr H, Wildhirt E, Ringelmann R, Hilfenhaus M, Reisert P M, Reitinger J, Burckhardt J, Seelig H P
Institut für Immunologie und Experimentelle Pathologie GmbH, Karlsruhe.
Immun Infekt. 1988 Jun;16(3):85-90.
A hepatitis non-A,non-B-associated substance (HNANB-AS) excreted in feces has been detected by means of a sandwich radioimmunoassay using reconvalescent serum and IgG from patients with posttransfusion HNANB. 4380 stool filtrates from 1599 patients were screened with this assay. In patients with posttransfusion or sporadic acute and chronic HNANB the substance was detected with a mean frequency of 34%, in acute posttransfusion HNANB, where samples were screened at the beginning of the clinical symptoms, 71.4% of stool specimens were positive for HNANB-AS. A high frequency of positive results was found in patients undergoing dialysis (31.3%), hemophiliacs (16.6%) and patients with cryptogenic cirrhosis (16%). Positive specimens were seen in hepatitis A (7.1%), acute and chronic HBV-infection (9.6%), various liver diseases (7.8%), outpatients of a physician (3.1%) and clinical or laboratory staff (6%). In these groups, too, anamnestic data speaking in favour for a possible HNANB frequently were obtained. The discovery of a partially double-stranded circular DNA of 5.0 Kb in HNANB-AS which sequence differs from human, bacterial and known viral DNA, argues the presence of a viral particle in stools of patients with sporadic and parenteral HNANB.
采用来自输血后非甲非乙型肝炎（HNANB）患者的恢复期血清和IgG，通过夹心放射免疫分析法检测出粪便中排泄的一种非甲非乙型肝炎相关物质（HNANB-AS）。用该分析法对1599例患者的4380份粪便滤液进行了筛查。在输血后或散发性急慢性HNANB患者中，该物质的检出频率平均为34%；在输血后急性HNANB患者中，于临床症状开始时进行样本筛查，71.4%的粪便标本HNANB-AS呈阳性。在接受透析的患者（31.3%）、血友病患者（16.6%）和隐源性肝硬化患者（16%）中发现了较高频率的阳性结果。在甲型肝炎患者（7.1%）、急慢性乙肝感染患者（9.6%）、各种肝病患者（7.8%）、内科门诊患者（3.1%）以及临床或实验室工作人员（6%）中也发现了阳性标本。在这些组中，也经常获得支持可能感染HNANB的既往史数据。在HNANB-AS中发现了一段5.0 Kb的部分双链环状DNA，其序列不同于人类、细菌和已知病毒的DNA，这表明散发性和经肠道外感染HNANB患者的粪便中存在病毒颗粒。
