Bray R A, Landay A L
Department of Immunology/Microbiology, Rush-Presbyterian-St Luke's Medical Center, Chicago, Ill.
Arch Pathol Lab Med. 1989 Jun;113(6):579-90.
Flow cytometric technology has evolved tremendously over the past 5 years. Among the major advancements have been the development of monoclonal antibodies, fluorescent labels, and computer software that makes it possible to perform routine multiparameter analyses. The clinical application of such analyses requires that standards and quality control procedures be established. Areas that need to be specifically addressed include sample preparation, staining, instrument calibration, and sample and data analysis. This study describes the basic elements involved in establishing such procedures and explores the use of multiparameter flow cytometric analysis in the characterization of peripheral blood mononuclear cells. Two-color, flow cytometric analysis of T, B, and natural killer cells can provide important insights into the biologic features of these cells as well as significant diagnostic information.
在过去5年里，流式细胞术有了巨大的发展。主要进展包括单克隆抗体、荧光标记以及计算机软件的开发，这些使得进行常规多参数分析成为可能。此类分析的临床应用需要建立标准和质量控制程序。需要特别关注的领域包括样本制备、染色、仪器校准以及样本和数据分析。本研究描述了建立此类程序所涉及的基本要素，并探讨了多参数流式细胞术分析在外周血单个核细胞特征鉴定中的应用。对T细胞、B细胞和自然杀伤细胞进行双色流式细胞术分析，可以为这些细胞的生物学特性提供重要见解，以及重要的诊断信息。
