Shapiro H M
Arch Pathol Lab Med. 1989 Jun;113(6):591-7.
Flow cytometric DNA content analysis, employing fluorescent stains that bind stoichiometrically to DNA, is widely used by researchers and clinicians to monitor cell growth kinetics and to detect abnormalities in nuclear DNA content in tumor cells. Measurement precision is critical because small differences in DNA content may be significant; plastic spheres and cell nuclei are used for instrument calibration and standardization. Preparative techniques must provide a uniformly stained sample representative of the cells or tissue under investigation; multiparameter gating may facilitate cell selection within samples. Flow cytometry of light scattering, surface or intracellular antigens, RNA, chromatin structure, and/or DNA synthesis may provide additional useful information about neoplastic cell behavior.
采用与DNA进行化学计量结合的荧光染料的流式细胞术DNA含量分析，被研究人员和临床医生广泛用于监测细胞生长动力学以及检测肿瘤细胞核DNA含量的异常情况。测量精度至关重要，因为DNA含量的微小差异可能具有重要意义；塑料微球和细胞核用于仪器校准和标准化。制备技术必须提供一个能代表所研究细胞或组织的均匀染色样本；多参数门控有助于在样本中选择细胞。对光散射、表面或细胞内抗原、RNA、染色质结构和/或DNA合成进行流式细胞术检测，可能会提供有关肿瘤细胞行为的其他有用信息。
