Wu Deyao, Pan Huixing, Zhou Yunfeng, Zhou Jian, Fan Yuanfeng, Qu Ping
Department of Urology, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng First People's Hospital, Yancheng, Jiangsu 224001, P.R. China.
Department of Urology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Mol Med Rep. 2014 Jun;9(6):2491-8. doi: 10.3892/mmr.2014.2116. Epub 2014 Apr 4.
microRNA (miRNA)-133b has been revealed to be downregulated in head and neck/oral, bladder, human non-small cell lung, colorectal and esophageal squamous cell cancer. The present study examined the expression of miR-133b in renal cell carcinoma (RCC) cell lines and the effects of miRNA-133b on RCC cell proliferation, migration and invasion. Quantitative polymerase chain reaction was used to detect the expression of miR-133b in RCC cell lines. Following transfection of miR-133b, the expression of miR-133b was examined and a cell viability assay, cell migration assay, cell invasion assay, western blot analysis and luciferase assay were conducted in RCC cell lines. The present study revealed that miRNA‑133b was downregulated and inhibited cell proliferation, migration and invasion in 786-O and A498 cells. In addition, to the best of our knowledge, the present study provided the first evidence that miRNA-133b may directly target matrix metallopeptidase 9 (MMP-9) in RCC. The present study also provided evidence that miRNA-133b suppresses cell proliferation, migration and invasion by targeting MMP-9 in RCC cell lines. These results suggested that miRNA-133b may be used for the development of novel molecular markers and therapeutic approaches to inhibit the metastasis of RCC.
微小RNA（miRNA）-133b已被发现在头颈/口腔癌、膀胱癌、人非小细胞肺癌、结直肠癌和食管鳞状细胞癌中表达下调。本研究检测了miR-133b在肾细胞癌（RCC）细胞系中的表达，以及miRNA-133b对RCC细胞增殖、迁移和侵袭的影响。采用定量聚合酶链反应检测RCC细胞系中miR-133b的表达。转染miR-133b后，检测miR-133b的表达，并在RCC细胞系中进行细胞活力测定、细胞迁移测定、细胞侵袭测定、蛋白质免疫印迹分析和荧光素酶测定。本研究表明，miRNA-133b在786-O和A498细胞中表达下调，并抑制细胞增殖、迁移和侵袭。此外，据我们所知，本研究首次提供证据表明，miRNA-133b可能在RCC中直接靶向基质金属蛋白酶9（MMP-9）。本研究还提供证据表明，miRNA-133b通过靶向RCC细胞系中的MMP-9抑制细胞增殖、迁移和侵袭。这些结果表明，miRNA-133b可用于开发新型分子标志物和治疗方法，以抑制RCC的转移。
