Wu Deyao, Li Min, Wang Linmao, Zhou Yunfeng, Zhou Jian, Pan Huixing, Qu Ping
Department of Urology, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu 224001, P.R. China.
Department of Thoracic Surgery, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu 224001, P.R. China.
Mol Med Rep. 2014 Jul;10(1):393-8. doi: 10.3892/mmr.2014.2149. Epub 2014 Apr 15.
microRNA‑145 (miR‑145) has been reported to be frequently downregulated in various types of cancer, including renal, prostate, bladder, lung and colon cancer, as well as B‑cell malignancies. The present study examined the effects of miR‑145 on the cell proliferation, migration and invasion of renal cell carcinoma (RCC). Following transfection of miR‑145, an MTT, cell migration, cell invasion and luciferase assays, and western blot analysis were conducted in RCC cell lines. The present study demonstrated that miR‑145 inhibited cell proliferation, migration and invasion in 786‑O and A498 cells. The present study also demonstrated for the first time, to the best of our knowledge, that miR‑145 may directly target matrix metallopeptidase‑11 (MMP‑11) in RCC. miR‑145 was demonstrated to suppress cell proliferation, migration and invasion by targeting MMP‑11 in RCC cell lines. These results suggested that it may be investigated as a predictive marker for the early detection of tumor metastasis and for targeting therapeutic drugs to inhibit the invasion of RCC.
据报道，微小RNA-145（miR-145）在包括肾癌、前列腺癌、膀胱癌、肺癌和结肠癌以及B细胞恶性肿瘤在内的各种类型癌症中经常下调。本研究检测了miR-145对肾细胞癌（RCC）细胞增殖、迁移和侵袭的影响。在RCC细胞系中转染miR-145后，进行了MTT、细胞迁移、细胞侵袭和荧光素酶测定以及蛋白质印迹分析。本研究表明，miR-145抑制786-O和A498细胞的增殖、迁移和侵袭。据我们所知，本研究还首次证明miR-145可能在RCC中直接靶向基质金属肽酶-11（MMP-11）。在RCC细胞系中，miR-145通过靶向MMP-11抑制细胞增殖、迁移和侵袭。这些结果表明，它可作为肿瘤转移早期检测的预测标志物以及靶向治疗药物以抑制RCC侵袭进行研究。
