恰加斯病患者体内的抗α-半乳糖基抗体。可能的生物学意义。 - Suppr | 超能文献

恰加斯病患者体内的抗α-半乳糖基抗体。可能的生物学意义。

Anti-alpha-galactosyl antibodies in chagasic patients. Possible biological significance.

Milani S R, Travassos L R

Disciplina de Micologia, Escola Paulista de Medicina, São Paulo, Brasil.

Braz J Med Biol Res. 1988;21(6):1275-86.

Abstract

Antibodies from Chagasic patients (Ch), bound to a column of immobilized purified glycoprotein 25-kDa antigen of Trypanosoma cruzi epimastigotes (gp25), were partially lytic (70% lysis at 50 micrograms/ml) to cultured metacyclic trypomastigotes (Y strain) in a complement-mediated reaction (alternative pathway). 2. Antibodies (Ch) eluted with galactose from a melibiose-Agarose column reacted in ELISA titration plates with gp25, whole cells of metacyclic trypomastigotes (Y strain), and less intensely with aqueous extracts of either T. cruzi (CL-14 strain) or Herpetomonas muscarum. 3. Agglutination of rabbit erythrocytes by anti-Gal antibodies from Chagasic patients or normal human sera (NHS) was inhibited by galactose and alpha-but not beta-anomeric galactosides. 4. Anti-Gal antibodies (Ch or NHS) were lytic to metacyclic trypomastigotes in complement-mediated reactions. 5. Agglutination of rabbit red cells by a serum sample from a T. cruzi-infected Rhesus monkey was inhibited by laminin. This serum was also lytic to metacyclic forms of T. cruzi (Y strain) involving the alternative complement pathway (70% lysis at 1:20 final dilution). 6. These results open the possibility of using anti-Gal antibodies for protection against infection by virulent T. cruzi.

摘要

恰加斯病患者（Ch）的抗体与固定有克氏锥虫前鞭毛体纯化的25-kDa糖蛋白抗原（gp25）的柱结合后，在补体介导的反应（替代途径）中对培养的循环后期锥鞭毛体（Y株）具有部分溶细胞作用（50微克/毫升时70%的细胞溶解）。2. 用半乳糖从蜜二糖 - 琼脂糖柱上洗脱的抗体（Ch）在ELISA滴定板中与gp25、循环后期锥鞭毛体（Y株）的全细胞反应，与克氏锥虫（CL - 14株）或食蟹赫氏鞭毛虫的水提取物反应较弱。3. 恰加斯病患者或正常人血清（NHS）中的抗半乳糖抗体对兔红细胞的凝集作用受到半乳糖和α - 而非β - 异头半乳糖苷的抑制。4. 抗半乳糖抗体（Ch或NHS）在补体介导的反应中对循环后期锥鞭毛体具有溶细胞作用。5. 克氏锥虫感染的恒河猴的血清样本对兔红细胞的凝集作用受到层粘连蛋白的抑制。该血清对克氏锥虫（Y株）的循环后期形式也具有溶细胞作用，涉及替代补体途径（终末稀释度为1:20时70%的细胞溶解）。6. 这些结果开启了使用抗半乳糖抗体预防强毒株克氏锥虫感染的可能性。