大蒜素调节原代大鼠肝细胞的抗氧化和解毒能力。

Allicin modulates the antioxidation and detoxification capabilities of primary rat hepatocytes.

机构信息

Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan, Taiwan.

Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.

出版信息

J Tradit Complement Med. 2012 Oct;2(4):323-30. doi: 10.1016/s2225-4110(16)30118-3.

DOI:10.1016/s2225-4110(16)30118-3

PMID:24716147

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3942910/

Abstract

The effect of allicin, an active ingredient of garlic, on lactate dehydrogenase (LDH) leakage, lipid peroxidation, glutathione (GSH) content, and GSH-related enzyme activity was investigated in primary hepatocytes. In this study, allicin was synthesized in our laboratory as an experimental material, and primary hepatocytes isolated from Sprague-Dawley rats were used as an experimental model. According to the results, hepatocytes treated with 10 μM allicin did not differ from the control on LDH leakage during various incubation times. When the hepatocytes were treated with 10 μM allicin, their levels of thiobarbituric acid reactive-substances (TBARS) did not differ significantly from that of the control within the 8-h incubation. However, the TBARS values of hepatocytes treated with 30 and 50 μM allicin were higher compared to the control after incubation for 4 h and 8 h, respectively. The hepatocyte intracellular GSH content was significantly higher than that of the control after 30 μM allicin treatment, but treatment with 50 μM allicin caused a significant GSH depletion after incubation for 4 h or longer. In addition, when hepatocytes were treated for 24 h with 10 or 30 μM allicin, glutathione peroxidase (GPx) activity was significantly increased compared to that of the control, whereas 50 μM allicin treatment for 24 h or longer significantly decreased the GPx activity. Glutathione reductase (GRd) activity was significantly increased when the hepatocytes were treated with 10 μM allicin for 24 h, but GRd activity significantly decreased when the hepatocytes were treated with 50 μM allicin. However, hepatocytes treated for 24 h with 10 or 30 μM allicin showed significantly increased glutathione S-transferase (GST) activity compared to the control. These results suggest that 10 μM allicin potentially enhances the antioxidation and detoxification capabilities of primary rat hepatocytes.

摘要

大蒜中的活性成分蒜素对乳酸脱氢酶（LDH）渗漏、脂质过氧化、谷胱甘肽（GSH）含量和 GSH 相关酶活性的影响在原代肝细胞中进行了研究。在本研究中，我们实验室合成了蒜素作为实验材料，使用分离自 Sprague-Dawley 大鼠的原代肝细胞作为实验模型。结果表明，在不同孵育时间内，10μM 蒜素处理的肝细胞与对照组相比，LDH 渗漏无差异。当肝细胞用 10μM 蒜素处理时，其在 8 小时孵育时间内的硫代巴比妥酸反应物质（TBARS）水平与对照组无显著差异。然而，在用 30 和 50μM 蒜素处理的肝细胞中，分别在孵育 4 小时和 8 小时后，TBARS 值均高于对照组。用 30μM 蒜素处理后，肝细胞内 GSH 含量明显高于对照组，但孵育 4 小时或更长时间后，用 50μM 蒜素处理会导致 GSH 明显耗竭。此外，当用 10 或 30μM 蒜素处理肝细胞 24 小时时，谷胱甘肽过氧化物酶（GPx）活性与对照组相比显著增加，而用 50μM 蒜素处理 24 小时或更长时间时，GPx 活性显著降低。当用 10μM 蒜素处理肝细胞 24 小时时，谷胱甘肽还原酶（GRd）活性显著增加，但当用 50μM 蒜素处理时，GRd 活性显著降低。然而，用 10 或 30μM 蒜素处理 24 小时的肝细胞与对照组相比，谷胱甘肽 S-转移酶（GST）活性显著增加。这些结果表明，10μM 蒜素可能增强了原代大鼠肝细胞的抗氧化和解毒能力。