Cancer Control Laboratory of Cancer Institute and General Surgery, The First Affiliated Hospital of China Medical University, Shenyang 110001, PR China.
Oncol Rep. 2010 Dec;24(6):1585-92. doi: 10.3892/or_00001021.
Allicin is an active compound derived from garlic that has been shown to have antitumor properties in vitro. The current study was designed to explore the effects and the underlying mechanism of allicin on gastric cancer cells. The MTT assay was used to detect cell viability. Transmission electron microscopy, Rh123 and propidium iodide staining, annexin V/FITC assay and the mitochondrial membrane potential were used to assess for the presence of apoptosis. Immunocytochemistry, western blot analysis, and Q-RT-PCR were used to detect gene expression. We found that allicin reduced cell viability in a dose- and time-dependent manner, partly through induction of apoptosis in gastric cancer cells. At the molecular level, allicin induced cytochrome c release from the mitochondria and increased caspase-3, -8, and -9 activation, with concomitant upregulation of bax and fas expression in the tumor cells. Allicin treatment inhibited proliferation and induced apoptosis in SGC-7901 cancer cells. Both intrinsic mitochondrial and extrinsic Fas/FasL-mediated pathways of apoptosis occur simultaneously in SGC-7901 cells following allicin treatment. Data from the current study demonstrated that allicin should be further investigated as a novel cancer preventive or therapeutic agent in control of gastric cancer, with potential uses in other tumor types.
大蒜素是一种从大蒜中提取的活性化合物，已被证明具有体外抗肿瘤特性。本研究旨在探讨大蒜素对胃癌细胞的作用及其作用机制。MTT 法检测细胞活力。透射电镜、Rh123 和碘化丙啶染色、Annexin V/FITC 检测和线粒体膜电位用于评估细胞凋亡的存在。免疫细胞化学、Western blot 分析和 Q-RT-PCR 用于检测基因表达。结果发现，大蒜素呈剂量和时间依赖性降低细胞活力，部分通过诱导胃癌细胞凋亡。在分子水平上，大蒜素诱导线粒体中细胞色素 c 的释放，并增加 caspase-3、-8 和 -9 的激活，同时肿瘤细胞中 bax 和 fas 的表达上调。大蒜素处理抑制 SGC-7901 癌细胞的增殖并诱导其凋亡。在大蒜素处理后，SGC-7901 细胞中同时发生内在线粒体和外在 Fas/FasL 介导的凋亡途径。本研究数据表明，大蒜素应作为一种新型的癌症预防或治疗药物，进一步研究其在控制胃癌中的作用，在其他肿瘤类型中也有潜在的应用价值。
