Saukko P, Knight B
Department of Forensic Medicine, University of Oulu, Finland.
Forensic Sci Int. 1989 Mar;40(3):285-90. doi: 10.1016/0379-0738(89)90187-4.
The diagnostic significance of the eosin-fluorescence method was evaluated and compared with the enzymehistochemical beta-hydroxybutyrate-dehydrogenase method (beta-HBDH) and the degree of hyperchromasia in 568 samples from 24 bets-HBDH-negative and beta-HBDH-positive sudden cardiac deaths (SCD) and 23 non-cardiac deaths as controls. The effect of autolysis was investigated separately on isolated human hearts at +4 degrees C and +22 degrees C. All samples were examined without any knowledge of clinical or autopsy data. Normal olive-green fluorescence was observed in only five individuals of the control group and two in the beta-HBDH-negative one. The frequency of the yellow-fluorescence and hyperchromasia increased from the control subjects towards the beta-HBDH-positive-ones. The difference in frequency distribution of fluorescence between the groups was statistically highly significant (P less than 0.001). The change in fluorescence did not correlate with the loss of beta-HBDH-reaction or the distribution pattern of hyperchromasia. Postmortem autolysis did not change the fluorescence significantly but the 78% wrong positive subjects in the control group render this method too sensitive and unreliable for medicolegal purposes.
对嗜酸性荧光法的诊断意义进行了评估，并与酶组织化学β-羟丁酸脱氢酶法（β-HBDH）以及568份样本的染色过深程度进行了比较，这些样本来自24例β-HBDH阴性和β-HBDH阳性的心源性猝死（SCD）以及23例作为对照的非心源性死亡。分别在4℃和22℃下对离体人类心脏研究了自溶的影响。所有样本在不了解临床或尸检数据的情况下进行检查。仅在对照组的5例个体以及β-HBDH阴性组的2例个体中观察到正常的橄榄绿色荧光。从对照组到β-HBDH阳性组，黄色荧光和染色过深的频率增加。各组之间荧光频率分布的差异在统计学上具有高度显著性（P＜0.001）。荧光变化与β-HBDH反应的丧失或染色过深的分布模式无关。死后自溶并未使荧光发生显著改变，但对照组中78%的假阳性个体使得该方法对于法医学目的而言过于敏感且不可靠。
