Hirvonen J, Hiltunen J K, Saukko P
Department of Forensic Medicine, University of Oulu, Finland.
Forensic Sci Int. 1987 Dec;35(4):231-6. doi: 10.1016/0379-0738(87)90094-6.
To evaluate the diagnostic role of histochemically demonstrated aerobic dehydrogenases in ischemic myocardial injury NADH-diaphorase, succinate dehydrogenase (SDH), beta-hydroxybutyrate dehydrogenase (HBDH) and malate dehydrogenase (MDH) were demonstrated histochemically and the corresponding enzyme activities were measured biochemically in isolated perfused rats hearts after global ischemia from 0 to 12 h. The present data show that the enzyme-histochemical methods when used properly are more sensitive indicators of early ischemic injury than classical histological staining procedures. From the enzymes tested here the histochemical demonstration of HBDH turned out to be best suited for use when suspecting ischemic myocardial injury at autopsy.
为评估组织化学显示的需氧脱氢酶在缺血性心肌损伤中的诊断作用，对烟酰胺腺嘌呤二核苷酸黄递酶、琥珀酸脱氢酶（SDH）、β-羟丁酸脱氢酶（HBDH）和苹果酸脱氢酶（MDH）进行了组织化学显示，并在全心缺血0至12小时后的离体灌注大鼠心脏中对相应的酶活性进行了生化测定。目前的数据表明，当正确使用时，酶组织化学方法比传统的组织学染色程序更能敏感地指示早期缺血性损伤。在这里测试的酶中，HBDH的组织化学显示结果表明，在尸检怀疑有缺血性心肌损伤时最适合使用。
