Hristu Radu, Tranca Denis E, Stanciu Stefan G, Gregor Maros, Plecenik Tomas, Truchly Martin, Roch Tomas, Tofail Syed A M, Stanciu George A
1 Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania.
2 Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48 Bratislava, Slovakia.
Microsc Microanal. 2014 Apr;20(2):586-95. doi: 10.1017/S1431927614000191. Epub 2014 Apr 9.
The surface properties of hydroxyapatite, including electric charge, can influence the biological response, tissue compatibility, and adhesion of biological cells and biomolecules. Results reported here help in understanding this influence by creating charged domains on hydroxyapatite thin films deposited on silicon using electron beam irradiation and investigating their shape, properties, and carbon contamination for different doses of incident injected charge by two methods. Photoluminescence laser scanning microscopy was used to image electrostatic charge trapped at pre-existing and irradiation-induced defects within these domains, while phase imaging in atomic force microscopy was used to image the carbon contamination. Scanning Auger electron spectroscopy and Kelvin probe force microscopy were used as a reference for the atomic force microscopy phase contrast and photoluminescence laser scanning microscopy measurements. Our experiment shows that by combining the two imaging techniques the effects of trapped charge and carbon contamination can be separated. Such separation yields new possibilities for advancing the current understanding of how surface charge influences mediation of cellular and protein interactions in biomaterials.
羟基磷灰石的表面性质，包括电荷，会影响生物反应、组织相容性以及生物细胞和生物分子的黏附。本文报道的结果通过使用电子束辐照在沉积于硅上的羟基磷灰石薄膜上创建带电区域，并通过两种方法研究不同剂量入射注入电荷下它们的形状、性质和碳污染情况，有助于理解这种影响。光致发光激光扫描显微镜用于对捕获在这些区域内预先存在的和辐照诱导的缺陷处的静电荷进行成像，而原子力显微镜中的相成像用于对碳污染进行成像。扫描俄歇电子能谱和开尔文探针力显微镜用作原子力显微镜相衬和光致发光激光扫描显微镜测量的参考。我们的实验表明，通过结合这两种成像技术，可以分离捕获电荷和碳污染的影响。这种分离为推进当前对表面电荷如何影响生物材料中细胞和蛋白质相互作用介导的理解带来了新的可能性。
