McNeill D L, Westlund K N, Coggeshall R E
Department of Anatomical Sciences, University of Oklahoma, Oklahoma City 73190.
J Histochem Cytochem. 1989 Jul;37(7):1047-52. doi: 10.1177/37.7.2471724.
The present study demonstrates calcitonin gene-related peptide (CGRP), somatostatin (SOM), bombesin (BOM), and substance P (SP) at the electron microscopic level in lumbar dorsal root axons of normal rats. The highest percentages of labeled axons were for CGRP (14%) and then, in descending order, for SP (8.6%), SOM (6.8%), and BOM (3.1%). The labeled axons were exclusively unmyelinated for SP, SOM, and BOM, and predominantly unmyelinated for CGRP. These data are consistent with the data for labeled sensory cell bodies for these same compounds. We emphasize that these peptides were immunocytochemically visualized in the dorsal roots without experimental manipulation, such as colchicine or dorsal root ligation. Quantitative sampling of this type can be used to assay changes in response to physiological stimuli in numbers of sensory axons that contain identifiable concentrations of these peptides.
本研究在电子显微镜水平上展示了正常大鼠腰段背根轴突中的降钙素基因相关肽（CGRP）、生长抑素（SOM）、蛙皮素（BOM）和P物质（SP）。标记轴突比例最高的是CGRP（14%），然后依次是SP（8.6%）、SOM（6.8%）和BOM（3.1%）。对于SP、SOM和BOM，标记轴突均为无髓鞘轴突，而对于CGRP，标记轴突主要是无髓鞘轴突。这些数据与这些相同化合物标记感觉细胞体的数据一致。我们强调，这些肽在未经秋水仙碱或背根结扎等实验操作的情况下，在背根中通过免疫细胞化学方法得以可视化。这种类型的定量采样可用于测定含有可识别浓度这些肽的感觉轴突数量对生理刺激的反应变化。
