Gibbins I L, Wattchow D, Coventry B
Brain Res. 1987 Jun 23;414(1):143-8. doi: 10.1016/0006-8993(87)91335-7.
Double labelling immunofluorescence has shown two populations of unmyelinated sensory axons in human skin that contain immunoreactivity (IR) to calcitonin gene-related peptide (CGRP). One population also contains IR to substance P (SP), whilst the other also contains IR to somatostatin (SOM). Axons containing both CGRP-IR and SOM-IR comprised more than 75% of CGRP-IR axons associated with the epidermis; the rest of the CGRP-IR axons contained SP-IR. No axons contained both SP-IR and SOM-IR. Some dermal blood vessels were surrounded by axons containing both CGRP-IR and SP-IR, but most CGRP-IR perivascular axons contained SOM-IR without SP-IR. Sweat glands were well supplied with sensory axons containing CGRP-IR and weak SOM-IR but not SP-IR. Therefore, CGRP is a histochemical marker for a larger number of unmyelinated cutaneous afferents in human skin than is SP. CGRP itself may have a role in the mediation of responses to stimulation of at least two populations of sensory axons.
双重免疫荧光标记显示，人类皮肤中有两类无髓鞘感觉轴突，它们对降钙素基因相关肽（CGRP）具有免疫反应性（IR）。一类轴突对P物质（SP）也有免疫反应性，而另一类对生长抑素（SOM）有免疫反应性。同时含有CGRP免疫反应性和SOM免疫反应性的轴突占与表皮相关的CGRP免疫反应性轴突的75%以上；其余的CGRP免疫反应性轴突含有SP免疫反应性。没有轴突同时含有SP免疫反应性和SOM免疫反应性。一些真皮血管被同时含有CGRP免疫反应性和SP免疫反应性的轴突包围，但大多数血管周围的CGRP免疫反应性轴突含有SOM免疫反应性而没有SP免疫反应性。汗腺有丰富的含有CGRP免疫反应性和弱SOM免疫反应性但没有SP免疫反应性的感觉轴突供应。因此，与SP相比，CGRP是人类皮肤中更多无髓鞘皮肤传入神经的组织化学标记物。CGRP本身可能在介导至少两类感觉轴突对刺激的反应中发挥作用。
