Hayashi Y, Takemura T, Hirokawa K
Department of Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
J Histochem Cytochem. 1989 Jul;37(7):1147-52. doi: 10.1177/37.7.2471726.
The immunoreactivity of anti-neuron-specific enolase (NSE) and anti-Leu-7 on formalin-fixed sections of human fetal salivary gland epithelium was determined by the avidin-biotin-peroxidase complex (ABC) method. In addition, expression of some neuropeptides such as vasoactive intestinal polypeptide (VIP), somatostatin (SRIF), and substance P in the human salivary gland epithelium during the gestational period was observed, whereas the other polypeptides examined, including glucagon, cholecystokinin (CCK), Leu-enkephalin, and calcitonin were absent. NSE and Leu-7 immunoreactivity in the fetal salivary gland epithelium was observed solitarily or in groups commonly restricted to the developing duct epithelium. Positive immunoreactivity was observed in 46 cases with NSE (73%) and 44 cases with Leu-7 (70%) in 63 fetal salivary glands examined. In contrast, the incidence of positive cases stained with neuropeptides was lower than those of NSE and Leu-7 immunoreactivity in the human fetal salivary gland epithelium. These findings indicate that certain neuropolypeptides, as well as VIP, SRIF, and substance P present in the human fetal salivary gland epithelium may play a significant role in the development of the gland.
采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，测定抗神经元特异性烯醇化酶（NSE）和抗Leu-7在人胎儿涎腺上皮福尔马林固定切片上的免疫反应性。此外，观察了妊娠期人涎腺上皮中一些神经肽如血管活性肠肽（VIP）、生长抑素（SRIF）和P物质的表达情况，而所检测的其他多肽，包括胰高血糖素、胆囊收缩素（CCK）、亮氨酸脑啡肽和降钙素均未检测到。在胎儿涎腺上皮中，NSE和Leu-7免疫反应性单独或成群出现，通常局限于发育中的导管上皮。在63个检测的胎儿涎腺中，46例（73%）NSE呈阳性免疫反应，44例（70%）Leu-7呈阳性免疫反应。相比之下，人胎儿涎腺上皮中神经肽染色阳性病例的发生率低于NSE和Leu-7免疫反应性的发生率。这些发现表明，人胎儿涎腺上皮中存在的某些神经多肽以及VIP、SRIF和P物质可能在腺体发育中起重要作用。
