Papadopoulos G C, Parnavelas J G, Cavanagh M E
Department of Anatomy, Veterinary School, University of Thessaloniki, Greece.
Brain Res. 1987 Sep 8;420(1):95-9. doi: 10.1016/0006-8993(87)90243-5.
The peroxidase-antiperoxidase immunohistochemical technique has been used to examine the co-existence of peptides within individual neurons of the rat visual cortex. Pairs of consecutive paraffin sections were stained alternately for 2 of the 4 peptides: somatostatin (SRIF), vasoactive intestinal polypeptide (VIP), cholecystokinin (CCK) and neuropeptide Y (NPY). Analysis revealed the co-existence of SRIF with VIP, CCK and NPY and between VIP and CCK. These results show that the co-localization of neuropeptides in cortical neurons is more widespread than previously demonstrated.
过氧化物酶-抗过氧化物酶免疫组织化学技术已被用于检测大鼠视皮层单个神经元内肽的共存情况。将连续的石蜡切片对4种肽中的2种进行交替染色：生长抑素（SRIF）、血管活性肠肽（VIP）、胆囊收缩素（CCK）和神经肽Y（NPY）。分析显示SRIF与VIP、CCK和NPY共存，以及VIP和CCK共存。这些结果表明，神经肽在皮层神经元中的共定位比先前证明的更为广泛。
