Hayashi Y, Deguchi H, Nakahata A, Kurashima C, Hirokawa K
Department of Pathology, Tokushima University School of Dentistry, Japan.
Pathobiology. 1990;58(4):212-20. doi: 10.1159/000163587.
The immunoreactivity of anti-neuron-specific enolase (NSE) and anti-Leu-7 on formalin-fixed sections of human salivary gland neoplasms was determined by the avidin-biotin-peroxidase complex method. In addition, neuropeptides, such as vasoactive intestinal polypeptide, somatostatin, and substance P, in human salivary gland neoplasms were expressed, whereas other polypeptides, including glucagon, cholecystokinin, leu-enkephalin and calcitonin, were absent. When 182 paraffin-embedded examples of human salivary gland tumors, including 112 benign and 70 malignant neoplasms, were examined immunohistochemically, positive immunoreactivity was observed in: 51 cases with NSE (59%) and 46 cases with Leu-7 (54%) of 86 pleomorphic adenomas; 11 cases with Leu-7 (61%) of 18 Warthin's tumors; 7 cases with Leu-7 (58%) of 12 acinic cell carcinomas; 5 cases with NSE (31%) of 16 adenoid cystic carcinomas; 5 cases with NSE (42%) and 4 cases with Leu-7 (33%) of 12 adenocarcinomas; 4 cases with NSE (25%) and 6 cases with Leu-7 (38%) of 16 undifferentiated carcinomas. The other tumors, such as oxyphilic adenomas, basal cell adenomas, epidermoid carcinomas, and mucoepidermoid carcinomas, were nonreactive. Neuropeptides were observed in the neoplastic epithelial cells of certain tumors such as Warthin's tumors, acinic cell carcinomas, adenocarcinomas and undifferentiated carcinomas. These findings suggest the possibility that cells of neuroendocrine origin, present in certain neoplastic salivary gland epithelia may play a significant role in the histogenesis of human salivary gland neoplasms.
采用抗生物素蛋白-生物素-过氧化物酶复合物法，测定抗神经元特异性烯醇化酶（NSE）和抗Leu-7在人涎腺肿瘤福尔马林固定切片上的免疫反应性。此外，人涎腺肿瘤中表达了神经肽，如血管活性肠肽、生长抑素和P物质，而其他多肽，包括胰高血糖素、胆囊收缩素、亮脑啡肽和降钙素则未表达。当对182例石蜡包埋的人涎腺肿瘤标本进行免疫组织化学检查时，其中包括112例良性肿瘤和70例恶性肿瘤，结果显示：86例多形性腺瘤中，51例NSE阳性（59%），46例Leu-7阳性（54%）；18例沃辛瘤中，11例Leu-7阳性（61%）；12例腺泡细胞癌中，7例Leu-7阳性（58%）；16例腺样囊性癌中，5例NSE阳性（31%）；12例腺癌中，5例NSE阳性（42%），4例Leu-7阳性（33%）；16例未分化癌中，4例NSE阳性（25%），6例Leu-7阳性（38%）。其他肿瘤，如嗜酸性腺瘤、基底细胞腺瘤、表皮样癌和黏液表皮样癌均无反应。在某些肿瘤如沃辛瘤、腺泡细胞癌、腺癌和未分化癌的肿瘤上皮细胞中观察到神经肽。这些发现提示，某些涎腺肿瘤上皮中存在的神经内分泌起源细胞可能在人涎腺肿瘤的组织发生中起重要作用。
