Biggiogera M, Biggiogera F F
Centre de Microscopie Electronique de l'Université de Lausanne, Switzerland.
J Histochem Cytochem. 1989 Jul;37(7):1161-6. doi: 10.1177/37.7.2471727.
Ultra-thin sections of various tissues were stained with ethidium bromide or propidium iodide, two fluorescent markers widely used for quantitation of nucleic acids. The fluorochromes, tested at different concentrations, were then revealed by incubation of the sections with neutralized phosphotungstic acid. We showed that at the electron microscopic level only nucleic acid-containing structures are revealed. Chromatin, nucleolus, and ribosomes appear to be stained by the end-product of the reaction. Furthermore, controls with proteases and nucleases showed that the staining is related to the binding of the fluorochromes to DNA and RNA and to the subsequent detection of the dyes by neutralized PTA.
各种组织的超薄切片用溴化乙锭或碘化丙啶染色，这两种荧光标记物广泛用于核酸定量。然后，将不同浓度的荧光染料与中和的磷钨酸孵育切片后进行显色。我们发现，在电子显微镜水平上，只有含核酸的结构会显色。染色质、核仁以及核糖体似乎都被反应终产物染色。此外，蛋白酶和核酸酶对照实验表明，染色与荧光染料与DNA和RNA的结合以及随后用中和的磷钨酸检测染料有关。
