Ninomiya Kazuaki, Fukuda Aya, Ogino Chiaki, Shimizu Nobuaki
Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 920-1192, Japan.
Division of Natural System, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan.
Ultrason Sonochem. 2014 Sep;21(5):1624-8. doi: 10.1016/j.ultsonch.2014.03.010. Epub 2014 Mar 19.
In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. Subsequently, hydroxyl radicals were generated from the TiO2 NPs after activation by external ultrasound irradiation (TiO2/US treatment). Although 30% of the normal breast cells (human mammary epithelial cells) exhibited the uptake of avidin-modified TiO2 NPs, over 80% of the breast cancer cells (MCF-7) exhibited the uptake of avidin-TiO2 NPs. Next the effect of the TiO2/US treatment on MCF-7 cell growth was examined for up to 96 h after 1-MHz ultrasound was applied (0.1 W/cm(2), 30 s) to cells that incorporated the TiO2 NPs. No apparent cell injury was observed until 24h after the treatment, but the viable cell concentration declined to 68% compared with the control at 96 h.
在本研究中，我们基于递送用抗生物素蛋白修饰的二氧化钛（TiO₂）纳米颗粒（NPs），将声动力疗法应用于癌细胞，该纳米颗粒可优先区分癌细胞与健康细胞。随后，通过外部超声照射激活后，TiO₂ NPs产生了羟基自由基（TiO₂/超声处理）。虽然30%的正常乳腺细胞（人乳腺上皮细胞）表现出对抗生物素蛋白修饰的TiO₂ NPs的摄取，但超过80%的乳腺癌细胞（MCF-7）表现出对抗生物素蛋白-TiO₂ NPs的摄取。接下来，在对掺入TiO₂ NPs的细胞施加1-MHz超声（0.1 W/cm²，30 s）后，检测TiO₂/超声处理对MCF-7细胞生长长达96小时的影响。在处理后24小时之前未观察到明显的细胞损伤，但在96小时时，与对照组相比，活细胞浓度下降至68%。
