Lanier L L, Testi R, Bindl J, Phillips J H
Becton Dickinson Monoclonal Center, Inc., Mountain View, California 94043.
J Exp Med. 1989 Jun 1;169(6):2233-8. doi: 10.1084/jem.169.6.2233.
Neural cell adhesion molecule (N-CAM) is a membrane glycoprotein expressed on neural and muscle tissues that is involved in homotypic adhesive interactions. We have demonstrated that N-CAM also is expressed on hematopoietic cells, and is recognized by the anti-Leu-19 mAb. Leu-19 is preferentially expressed on NK cells and T lymphocytes that mediate MHC-unrestricted cytotoxicity, but is also present on some myeloid leukemia cell lines. On NK cells, T cells, the KG1a.5 hematopoietic cell line, and a neuroblastoma cell line, Leu-19 is a approximately 140-kD polypeptide with N-linked carbohydrates and abundant sialic acid residues. Sequential immunoprecipitation and peptide mapping demonstrated that the Leu-19 and N-CAM molecules expressed on leukocyte and neuroblastoma cell lines are similar structures. These findings suggest that the Leu-19 antigen on leukocytes may be involved in cell adhesion, analogous to the function on N-CAM on neural cells.
神经细胞黏附分子（N-CAM）是一种表达于神经和肌肉组织上的膜糖蛋白，参与同型黏附相互作用。我们已经证明N-CAM也在造血细胞上表达，并被抗Leu-19单克隆抗体识别。Leu-19优先表达于介导MHC非限制性细胞毒性的NK细胞和T淋巴细胞上，但也存在于一些髓系白血病细胞系中。在NK细胞、T细胞、KG1a.5造血细胞系和成神经细胞瘤细胞系上，Leu-19是一种约140-kD的多肽，带有N-连接碳水化合物和丰富的唾液酸残基。连续免疫沉淀和肽图谱分析表明，在白细胞和成神经细胞瘤细胞系上表达的Leu-19和N-CAM分子结构相似。这些发现提示，白细胞上的Leu-19抗原可能参与细胞黏附，类似于神经细胞上N-CAM的功能。
