Deguchi T, Matsui H, Ehara H, Kobayashi K, Saito I, Shinoda I, Takahashi Y, Kuriyama M, Ban Y, Kawada Y
Nihon Hinyokika Gakkai Zasshi. 1989 Mar;80(3):349-52. doi: 10.5980/jpnjurol1989.80.349.
Antigens recognized by anti-gamma-seminoprotein (gamma-Sm) antibody and anti-prostate-specific antigen (PA) antibody were analyzed immunologically by Ouchterlony method and Western blotting method. The double immunodiffusion test demonstrated that anti-gamma-Sm antibody and anti-PA antibody reacted against an identical antigen in seminal fluid or prostate homogenate. In the immunoblotting examination, anti-gamma-Sm antibody and anti-PA antibody formed a single and identical band, which gave a molecular weight of 33,000, in seminal fluid and prostate homogenate. This study demonstrated that anti-gamma-Sm antibody and anti-PA antibody recognized a single and identical antigen in seminal fluid and prostate homogenate and suggested that an identical material could be measured in sera of patients with prostate cancer by immunoassay systems using anti-gamma-Sm antibody and anti-PA antibody.
采用欧氏免疫扩散法和蛋白质印迹法，对抗γ-精蛋白（γ-Sm）抗体和抗前列腺特异性抗原（PA）抗体所识别的抗原进行了免疫学分析。双向免疫扩散试验表明，抗γ-Sm抗体和抗PA抗体与精液或前列腺匀浆中的同一抗原发生反应。在免疫印迹检测中，抗γ-Sm抗体和抗PA抗体在精液和前列腺匀浆中形成了一条单一且相同的条带，其分子量为33,000。本研究表明，抗γ-Sm抗体和抗PA抗体在精液和前列腺匀浆中识别单一且相同的抗原，并提示使用抗γ-Sm抗体和抗PA抗体的免疫分析系统可在前列腺癌患者血清中检测到相同物质。
