Bahreini Fatemeh, Soltanian Ali Reza, Mehdipour Parvin
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Pour Sina Avenue, 14176-13151, Tehran, Iran.
Department of Biostatistics and Epidemiology, Modeling of Noncommunicable Diseases Research Center, School of Public Health, Hamadan University of Medical Sciences, Shahid Fahmideh Street, P.O.Box 4171, 65155, Hamadan, Iran.
Breast Cancer. 2015 Nov;22(6):615-25. doi: 10.1007/s12282-014-0528-0. Epub 2014 Apr 10.
We performed this meta-analysis study to evaluate the concordance and discordance between immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in detecting HER2 alteration in human breast cancer.
As a meta-analysis, the present study evaluated the available data from previous studies on the HER2 gene detected by IHC and FISH. To indicate the meta-analysis results, a forest plot was used.
We identified 172 citations, for which our inclusion criteria were met by 18 articles, representing 6629 cases. The overall concordance and discordance rate between IHC staining with score 0/1+ and FISH for detection failure of HER2 expression was 96 and 4 %, respectively. The present study showed that the overall proportion of FISH positive and negative rate for IHC score 2+ for detection of HER2 expression was 36 and 64 %, respectively; and 91 and 9 % for 3+ IHC scores.
The results of this study show that IHC score 0/1+ and 3+ cannot be completely considered as negative and positive breast cancer test, respectively. Therefore, we suggest a valid and complementary test, the same as FISH, to explore HER2 expression.
我们开展这项荟萃分析研究，以评估免疫组织化学（IHC）和荧光原位杂交（FISH）在检测人类乳腺癌HER2改变方面的一致性和不一致性。
作为一项荟萃分析，本研究评估了先前关于通过IHC和FISH检测HER2基因的研究中的可用数据。为了展示荟萃分析结果，使用了森林图。
我们识别出172篇文献，其中18篇文章符合我们的纳入标准，代表6629例病例。IHC染色评分为0/1+且FISH检测HER2表达失败的总体一致性和不一致率分别为96%和4%。本研究表明，对于检测HER2表达，IHC评分为2+时FISH阳性和阴性率的总体比例分别为36%和64%；IHC评分为3+时分别为91%和9%。
本研究结果表明，IHC评分0/1+和3+不能分别完全视为乳腺癌检测的阴性和阳性结果。因此，我们建议采用一种有效的补充检测方法，与FISH相同，来探究HER2表达。
