Tan Yiqun, Liu Min, Gao Junkuo, Yu Jiancan, Cui Yuanjing, Yang Yu, Qian Guodong
State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Department of Materials Science & Engineering, Zhejiang University, Hangzhou, China.
Dalton Trans. 2014 Jun 7;43(21):8048-53. doi: 10.1039/c4dt00167b. Epub 2014 Apr 9.
A new fluorescent probe, (E)-3-(3-(4-([2,2':6',2''-terpyridin]-4'-yl)phenyl)acryloyl)-7-(diethylamino)-2H-chromen-2-one (ZC-F4), composed of coumarin as the fluorophore and terpyridine as the receptor was designed and synthesized. This probe showed good selectivity and sensitivity towards Zn(2+) even at the ppb level with significant variation of emission wavelength (more than 100 nm shifts) after combination with Zn(2+). One can observe that the emission colour changed from green to red. A Job's plot test suggested a 1 : 1 stoichiometry between ZC-F4 and Zn(2+), and the theoretical calculation based on density functional theory has been carried out to gain an insight into the sensing mechanism. Furthermore, imaging of Zn(2+) in cells was also performed to test its feasibility in biology. This fluorescence probe should be a promising candidate for applications in cell-imaging, environment protection, water treatment and safety inspection.
设计并合成了一种新型荧光探针（E）-3-（3-（4-（[2,2':6',2''-三联吡啶]-4'-基）苯基）丙烯酰基）-7-（二乙氨基）-2H-色烯-2-酮（ZC-F4），其由香豆素作为荧光团和三联吡啶作为受体组成。该探针即使在十亿分之一水平下对Zn(2+)也表现出良好的选择性和灵敏度，与Zn(2+)结合后发射波长有显著变化（超过100 nm位移）。可以观察到发射颜色从绿色变为红色。Job曲线测试表明ZC-F4与Zn(2+)之间的化学计量比为1:1，并基于密度泛函理论进行了理论计算以深入了解传感机制。此外，还进行了细胞中Zn(2+)的成像以测试其在生物学中的可行性。这种荧光探针有望应用于细胞成像、环境保护、水处理和安全检测。
