Wright G L, Beckett M L, Lipford G B, Haley C L, Schellhammer P F
Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk 23501.
Int J Cancer. 1991 Mar 12;47(5):717-25. doi: 10.1002/ijc.2910470516.
A prostate carcinoma-associated antigen recognized by MAb TURP-27 was characterized immunohistochemically and biochemically. TURP-27 antigen was found localized in the cell membrane and cytoplasm of the ductal epithelial cells of normal (10%), benign (75-100%) and malignant (20-100%) prostate cells. Fetal prostate tissues were also found to express the TURP-27 antigen, suggesting expression early in development. This antigen was not expressed by non-prostate tumors examined, but significant cross-reactivity was observed in myelinated nerves while minor cross-reactivity was seen in certain lymphocyte subsets, cells in the adrenal medulla and chief cells of stomach. Immunoblotting and biochemical data demonstrated that the TURP-27 antigen is a sialic-acid-containing glycoprotein complex with major molecular species in prostate tissues of 310-250, 180, 140, 115, 95-90, 69, and 40- to 35-kDa. Immunoblotting patterns similar to those observed for prostate tissues were also seen in CNS extracts with the exception of the 69 and 40- to 35-kDa proteins. This prostate carcinoma-associated sialoglycoprotein complex (PAC) recognized by MAb TURP-27 is likely to represent a novel tumor antigen expressed by prostate tumors.
通过免疫组织化学和生物化学方法对单克隆抗体TURP - 27识别的前列腺癌相关抗原进行了特性分析。发现TURP - 27抗原定位于正常（10%）、良性（75 - 100%）和恶性（20 - 100%）前列腺细胞的导管上皮细胞的细胞膜和细胞质中。还发现胎儿前列腺组织表达TURP - 27抗原，提示在发育早期就有表达。所检测的非前列腺肿瘤不表达该抗原，但在有髓神经中观察到明显的交叉反应，在某些淋巴细胞亚群、肾上腺髓质细胞和胃主细胞中观察到轻微交叉反应。免疫印迹和生化数据表明，TURP - 27抗原是一种含唾液酸的糖蛋白复合物，在前列腺组织中的主要分子种类为310 - 250、180、140、115、95 - 90、69以及40 - 35 kDa。除了69 kDa和40 - 35 kDa的蛋白质外，在中枢神经系统提取物中也观察到了与前列腺组织相似的免疫印迹模式。这种被单克隆抗体TURP - 27识别的前列腺癌相关唾液糖蛋白复合物（PAC）可能代表一种由前列腺肿瘤表达的新型肿瘤抗原。
