Zhang Rui, Xu Lei-Bo, Zeng Hong, Yu Xian-Huan, Wang Jie, Liu Chao
Hepatogastroenterology. 2013 Nov-Dec;60(128):2042-7.
BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) with bile duct tumor thrombi (BDTT) is a rare event. The Bmi1 gene has been reported to play important roles in cancer initiation and progression. In this study, the expression of Bmi1 in HCC with BDTT was investigated.
The expression of Bmi1 was examined immunohistochemically in HCC patients with BDTT (B+ group), HCC patients with vascular invasion (V+ group) and combined hepatocellular carcinoma and cholangiocarcinoma (C+ group), respectively. Besides, the Bmi1 mRNA level was investigated by real time PCR in the fresh samples obtained from 13 cases in B+ group, 10 cases in V+ group, and 6 cases in C+ group, respectively.
Immunohistochemical staining for Bmi1 showed Bmi1 was highly expressed in the B+ group in comparison with the C+ group and the V+ group, respectively. The Bmi1 mRNA level by real time PCR also showed that it was significantly up-regulated in B+ group compared with those of C+ group and V+ group, respectively. However, there was no statistically significant difference in Bmi1 levels between the subjects with vascular invasion and the subjects without vascular invasion.
Bmi1 might play important roles in the development of BDTT in HCC.
背景/目的：肝细胞癌（HCC）合并胆管癌栓（BDTT）是一种罕见情况。据报道，Bmi1基因在癌症的发生和发展中发挥重要作用。本研究调查了Bmi1在HCC合并BDTT中的表达情况。
分别采用免疫组织化学方法检测HCC合并BDTT患者（B+组）、伴有血管侵犯的HCC患者（V+组）以及肝细胞癌合并胆管癌患者（C+组）中Bmi1的表达。此外，通过实时PCR分别检测从B+组的13例、V+组的10例和C+组的6例患者获取的新鲜样本中Bmi1 mRNA水平。
Bmi1免疫组织化学染色显示，与C+组和V+组相比，Bmi1在B+组中高表达。实时PCR检测的Bmi1 mRNA水平也显示，与C+组和V+组相比，Bmi1在B+组中显著上调。然而，有血管侵犯的患者与无血管侵犯的患者之间Bmi1水平无统计学显著差异。
Bmi1可能在HCC合并BDTT的发生发展中起重要作用。
