Sun W L, Fan Y L
Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.
Sci Sin B. 1988 Oct;31(10):1213-21.
The expression of the determinant for the K99 antigen of Escherichia coli in Yersinia enterocolitica avirulent transconjugants D29(pFS239) and L15(pFS239) was confirmed by means of SDS-polyacrylamide gel electrophoresis, double immunodiffusion and Western blotting. The conditions for the expression of K99 antigen genes in Y. enterocolitica and in E. coli were compared. The K99 antigen protein expressed in Y. enterocolitica in the form of pili on the surface of the cells was discussed. This article offers the first description on the expression in Y. enterocolitica of gene from organism other than Yersinia spp.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、双向免疫扩散和蛋白质免疫印迹法，证实了无毒转接合子大肠杆菌耶尔森氏菌D29(pFS239)和L15(pFS239)中K99抗原的决定簇表达。比较了K99抗原基因在小肠结肠炎耶尔森氏菌和大肠杆菌中的表达条件。讨论了在小肠结肠炎耶尔森氏菌中以菌毛形式在细胞表面表达的K99抗原蛋白。本文首次描述了耶尔森氏菌属以外的生物体基因在小肠结肠炎耶尔森氏菌中的表达。
