Titenko B M, Emdina I A, Lebedeva S A, Burlakova O S, Roshchina N V
Mol Gen Mikrobiol Virusol. 1988 Aug(8):41-6.
The plasmids pCG86 and RP4elt coding for thermolabile enterotoxins of Escherichia coli (LT) were transferred in conjugation to Yersinia enterocolitica and Yersinia pseudotuberculosis cells. Both plasmids were stably inherited by the recipient cells. The elt genes of the toxins were expressed in Yersinia cells at the level comparable to the one registered in Escherichia coli cells. In the broth cultures of transconjugant cells the major part of LT toxin is bound with cells (74-97%). The obtained data may serve an experimental basis in favour of possibility of Ent+ strains of Yersinia enterocolitica and Yersinia pseudotuberculosis formation in nature and expediency of search and diagnosing of such strains.
编码大肠杆菌不耐热肠毒素（LT）的质粒pCG86和RP4elt通过接合转移至小肠结肠炎耶尔森菌和假结核耶尔森菌细胞中。两种质粒均被受体细胞稳定遗传。毒素的elt基因在耶尔森菌细胞中的表达水平与在大肠杆菌细胞中记录的水平相当。在转接合子细胞的肉汤培养物中，大部分LT毒素与细胞结合（74%-97%）。所获得的数据可为自然界中形成小肠结肠炎耶尔森菌和假结核耶尔森菌Ent+菌株的可能性以及寻找和诊断此类菌株的合理性提供实验依据。
