Firinu Davide, Barca Maria Pina, Lorrai Maria Maddalena, Perra Silvia, Cabras Stefano, Muggianu Emma, Di Martino Maria Luisa, Manconi Paolo Emilio, Del Giacco Stefano R
Unit of Internal Medicine, Allergy and Clinical Immunology, Department of Medical Sciences "M. Aresu", University of Cagliari , Monserrato , Italy .
Autoimmunity. 2014 Sep;47(6):389-94. doi: 10.3109/08916934.2014.906582. Epub 2014 Apr 10.
To assess whether the immune derangement previously observed in SAPHO syndrome could be linked to variations in blood TH1, TH2 or TH17 lymphocytes frequency. Seven SAPHO patients with a protracted course of the disease were studied ex-vivo for intracellular cytokines production by means of flow-cytometry and compared with matched groups of Psoriatic Arthritis patients and healthy controls. The Kruskal-Wallis test on the median of the three categories showed that there is a significant association between the TH17 levels and the category (p value = 0.02474). The mean and variance for the proportion of IL-17 producing CD4+ cells were compared between groups showing significant differences between SAPHO versus PsA subgroup (p = 0.05) and SAPHO versus healthy controls (p = 0.008). Interestingly, activation of TH17 axis, but not of TH1 and TH2, has been found, and can be observed both in patients with different activity of the disease or treated with different drugs. The TH17 increase in peripheral blood of our SAPHO subjects resembles the one recently found in patients with different AIDs. Novel therapeutic options in these patients may therefore include IL-17 blockade.
为评估先前在滑膜炎、痤疮、脓疱病、骨肥厚、骨髓炎综合征（SAPHO综合征）中观察到的免疫紊乱是否与血液中TH1、TH2或TH17淋巴细胞频率的变化有关。对7例病程迁延的SAPHO患者进行体外研究，通过流式细胞术检测细胞内细胞因子的产生，并与银屑病关节炎患者和健康对照的匹配组进行比较。对三类人群中位数进行的Kruskal-Wallis检验表明，TH17水平与类别之间存在显著关联（p值 = 0.02474）。比较各组间产生IL-17的CD4 +细胞比例的均值和方差，结果显示SAPHO组与银屑病关节炎亚组之间（p = 0.05）以及SAPHO组与健康对照之间（p = 0.008）存在显著差异。有趣的是，已发现TH17轴激活，但TH1和TH2未激活，并且在疾病活动程度不同或接受不同药物治疗的患者中均能观察到。我们的SAPHO受试者外周血中TH17升高类似于最近在不同自身免疫性疾病患者中发现的情况。因此，这些患者的新治疗选择可能包括IL-17阻断。
